11:30-14:30	Registration
14:30-14:40	Welcome and opening of the meeting
14:40-15:20	Lecture 1 Chair: Lucia Latella Philippos Mourikis (Paris Est University, France) A self-made quiescent niche of muscle stem cells
15:20-16:20	Session 1/a: Muscle function, muscle stem cells and regenerative medicine Chairs: Biliana Lozanoska-Ochser, Giosuè Annibalini Elena Ruggieri (Milan, Italy) High Mobility Group Box 1 recycling orchestrates regeneration in skeletal muscle Lorenza Esposito (Rome, Italy) Injury-experienced satellite cells retain long-term enhanced regenerative capacity Federica Esposito (Rome, Italy) Study of IntegrinB1 transported by FAPs derived extracellular vesicles in correcting asymmetric division of dystrophic satellite cells Carmen Santangelo (Chieti, Italy) Post-mortem human muscle satellite cells: understanding of fundamental regenerative mechanism
16:20-16:50	Coffee break
16:50-17:05	Technical talk (by Bio-Techne) Dissecting Muscles: from spatial biology to single cell characterization and Omics. The Bio-Techne approach
17:05-18:20	Session 1/b: Muscle function, muscle stem cells and regenerative medicine Chairs: Libero Vitiello, Emilie Venereau Amélie Vergnol (Paris, France) Regulation of CaVβ1 isoform expression in skeletal muscle Vincent Gache (SFM, Lyon, France) Microtubule detyrosination is essential for neuromuscular junction stability and muscle stem cell function Bruno Allard (SFM, Lyon, France) Zebrafish, an animal model with ultra-high-performance skeletal muscle E-C coupling Vladimir Krylov (Prague, Czech Republic) The effect of Sertoli cells on striated muscle regeneration in the new model of Xenopus tropicalis Peter Zammit (London, United Kingdom) Identifying Satellite Cell-opathies: neuromuscular disorders caused by muscle stem cell dysfunction
18:20-18:30	Poster blitz 1 (odd numbers)
19:00-20:30	AperiPoster - Poster Session 1 (odd numbers) and Aperitivo Umbro

9:00-9:40	Lecture 2 Chair: Sestina Falcone Dada Pisconti (College of Arts and Sciences, Stony Brook, NY, USA) A unifying mechanism regulating muscle stem cell quiescence entry, maintenance and exit
9:40-10:40	Session 2: Muscle plasticity, aging and exercise Chairs: Elena Barbieri, Danilo Bondi Eloisa Turco (Padua, Italy) Peroxisomes in skeletal muscle homeostasis Giulia Ferrarese (Padua, Italy) A new way of studying the muscular secretome in a prematurely aged model Andrea Bracaglia (Rome, Italy) Transcriptional and proteomic regulation in age-dependent progressive decline of skeletal muscle regenerative capacities Ugo Carraro (Padua, Italy) It's never too early, it's never too late to counteract muscle decay with the Full-Body In-Bed Gym
10:40-11:10	Coffee break
11:10-12:10	Session 3/a: Muscle wasting and cachexia Chairs: Nicoletta Filigheddu, Vanina Romanello Giulia Gentili (Perugia, Italy) Tumor or muscle RAGE: which one sustains cancer-induced cachexia? Mariam Zouhair (Rome, Italy) 3D bioengineered skeletal muscle tissue as a tool for testing therapeutics for cancer-associated cachexia Tommaso Raiteri (Novara, Italy) Vitamin D binding protein induces skeletal muscle atrophy and contributes to cancer-associated muscle wasting Andrea David Re Cecconi (Milan, Italy) The p97-Nploc4 ATPase complex plays a role in muscle atrophy during cancer and amyotrophic lateral sclerosis
12:10-12:25	Technical talk (by Biond) MUSbit: Towards functional and dynamic in vitro muscle models
12:30-13:30	Lunch and (only for participants registered to the Advanced Myology Update course) Roundtable Discussions with the Invited Speakers: P. Mourikis, D. Pisconti, S. Tajbakhsh, S. Tedesco "A career in science. Options and opportunities"
14:00	Bus departure to Perugia
15:00-18:00	Convention at Dept Medicine and Surgery, University of Perugia

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Convention CAUSES AND MECHANISMS OF MUSCLE ATROPHY From terrestrial disuse to Space flights Chairs: Antonio Musarò (Sapienza University of Rome, Italy) Guglielmo Sorci (University of Perugia, Italy)

15:00	Welcome from authorities
15:30	Marco Sandri (University of Padua, Italy) Causes and mechanisms of muscle atrophy
15:50	Paola Costelli (University of Turin, Italy) Cancer-associated muscle atrophy
16:10	Sestina Falcone (Myology Research Centre, Paris, France) Age-related muscle atrophy
16:30	Stefania Fulle (University of Chieti-Pescara, Italy) Muscle atrophy. Lessons from Space
16:50	Paolo Nespoli (former ASI/ESA astronaut) My experience in Space
17:20	General discussion
18:00	Conclusions
The convention will be in Italian with simultaneous translation into English. A live streaming connection will be activated.

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18:30

Guided tour of Casa del Cioccolato Perugina and AperiChocolate

9:00-9:40	Lecture 3 Chair: Anna Urciuolo Shahragim Tajbakhsh (Institut Pasteur, Paris, France) Skeletal muscle stem and niche cell dynamics in developmental and regenerative myogenesis
9:40-10:40	Session 3/b: Muscle wasting and cachexia Chairs: Bert Blaauw, Valentina Saccone Noora Pöllänen (Helsinki, Finland) NAD+ depletion as a disease mechanism and therapy target for cancer cachexia Davide Acquarone (Turin, Italy) The chaperone protein Melusin counteracts muscle atrophy induced by cancer cachexia Giorgia Careccia (Milan, Italy) Unravelling new functions of Nfix as a new player in cancer cachexia Enrico Pierantozzi (Siena, Italy) Deletion of the muscle-specific internal promoter of the Ank1 gene results in a pre-diabetic phenotype
10:40-11:10	Coffee break
11:10-12:40	Session 4/a: Modeling and treating muscle diseases Chairs: Cesare Gargioli, Chiara Mozzetta Quentin Giraud (Illkirch-Graffenstaden, France) MTM1 overexpression efficiently rescues BIN1-related centronuclear myopathy Laura Pérez i Guàrdia (Illkirch-Graffenstaden, France) ORAI1 inhibition as an efficient preclinical therapy for tubular aggregate myopathy (TAM) and Stormorken syndrome (STRMK) Nefele Giarratana (Leuven, Belgium) Targeting rhabdomyosarcoma cancer progression by MICAL2 regulation Lucas Duvert (Marseille, France) Laser-assisted bio-printing and structuring for muscle modeling Beatrice Auletta (Padua, Italy) Native extracellular matrix support human neuromuscular organoid morphogenesis and function Capucine Trollet (SFM, Paris, France) Investigating the molecular, cellular and mechanistic differences of muscle fibrosis in human myopathies to determine to what extent fibrosis is a unique feature or results from different contributing factors
12:40-12:50	Poster blitz 2 (even numbers)
13:00-14:30	AperiPoster - Poster Session 2 (even numbers) and lunch
14:30-15:10	Lecture 4 Chair: Valeria Runfola Saverio Tedesco (University College London, United Kingdom) Engineering human skeletal muscle for advanced tissue, disease, and therapy modelling
15:10-16:25	Session 4/b: Modeling and treating muscle diseases Chairs: Graziella Messina, Alessandro Fanzani Beatrice Biferali (Milan, Italy) Immunopathogenesis of Facioscapulohumeral Muscular Dystrophy Sora Han (Seoul, South Korea) CTRP1, a myogenic protein in skeletal muscle differentiation and mitochondrial function Michela Gloriani (Rome, Italy) Beta -Sarcoglycanopathy (LGMDR4): a study of muscle diversity using a Sgcb KO mouse model Giovanni Delli Carpini (Rome, Italy) Gold nanoparticles conjugated to antisense oligonucleotides (ASOs) for targeted exon skipping in Duchenne muscular dystrophy Clara De Palma (Milan, Italy) SRT2104, a new SIRT1 activator, is an effective metabolic enhancer that promotes muscle recovery in DMD
16:25-16:55	Coffee break
16:55-17:55	Session 4/c: Modeling and treating muscle diseases Chairs: Roberta Sartori, Luca Madaro Giorgia Cavioli (Rome, Italy) Unveiling the HDAC4 functions in mediating the cross-talk between skeletal muscle fibers and fibro-adipogenic progenitors in Duchenne Muscular Dystrophy Francesca De Paolis (Rome, Italy) In vitro modeling of the human neuromuscular junction in a microfluidic device for the study of facioscapulohumeral dystrophy Anne Forand (Paris, France) Assessment of cardiac structure and function in a Dys-/-;Utr-/- mouse model of DMD treated with long term dystrophin replacement therapies Ilaria Zito (Parent Project, Rome, Italy) The voice of patients affected by Duchenne and Becker muscular dystrophy
18:00-19:00	IIM General meeting Giorgio Fanò-Illic (Free University of Alcatraz, Italy)  20 years of IIM: no ifs, no buts
20:00	Social Dinner - Awards and prizes
22:00	80’s-90’s Dance party with Tony Ross Dee Jay

High Training Course in ADVANCED MYOLOGY UPDATE 2023

10:00	Bus departure to Palazzo Bernabei
10:30-12:30	Dedicated lessons Philippos Mourikis New single-cell transcriptomics technologies and their relevance to muscle research Saverio Tedesco Development of new in vitro models of muscle tissue Shahragim Tajbakhsh Temporal evaluation of myogenic cell states Dada Pisconti How to design a translationally meaningful pre-clinical trial for muscle disorders
12:30	Light lunch

Posters always on display during the meeting

Discussions: ODD numbers – Thursday, 12 October (19:00-20:30)
EVEN numbers – Saturday, 14 October (13:00-14:30)

P.1  Vittoria Marini (Leuven, Belgium)
3D human cardiac models of Duchenne cardiomyopathy generation and NOX4 inhibition

P.2  Adriano Ciena (Rio Claro, Brazil)
Telocytes morphology at the myotendinous junction after muscle injury

P.3  Delia Verucci (Chieti, Italy)
Proteomic studies highlight a fundamental role of GAP43 for the correct functioning of cardiomyocytes

P.4  Gaia Giuriato (Verona, Italy)
Intrinsic Skeletal muscle contractility and Nrf2 in humans

P.5  Danilo Bondi (Chieti, Italy)
A pilot application of integrated procedures to accelerate, deepen, and guide genetic investigation on the myokinome

P.6  Elisabetta Meacci (Florence, Italy)
Sphingosine-1-phosphate/sphingosine-1-phosphate receptor (S1P/S1PR) axis modulates irisin signaling in skeletal muscle cells

P.7  Ozlem Kartal (Padua, Italy)
Targeting mitochondrial calcium and metabolism by RNA-based therapy in sarcopenia

P.8  Anna Pedrinolla (Trento, Italy)
Effect of high-intensity exercise training combined with dark chocolate rich in polyphenols and vitamin-E on muscle mass and strength in elderly people with dementia. Preliminary results from the Choko-Age study

P.9  Lorenzo Marramiero (Chieti, Italy)
Extracellular 500 μM GTP enhances myogenesis inducing specific gene and protein pathways

P.10  Giorgia Piccoli (Padua, Italy)
The regulation of protein synthesis and function in muscle wasting

P.11  Francesco Millozzi (Rome, Italy)
N6-methyladenosine (m6A) as a modulator of satellite cells function and skeletal muscle regeneration

P.12  Jurandyr Pimentel Neto (Rio Claro, Brazil)
Muscle injury protocol and adjacent cells at the neuromuscular junction region: a new aspect view

P.13  Emanuele Mocciaro (Milan, Italy)
MENIN is required for DUX4 expression and its pathological effects in FSHD muscular dystrophy

P.14  Martina Biglietto (Rome, Italy)
Engineered exosomes as a therapeutic tool to counteract muscle degeneration

P.15  Veronica Ruggieri (Rome, Italy)
The emerging role of polyamine pathway in skeletal muscle during ALS progression

P.16  Desiree Genovese (Rome, Italy)
Characterization of ex-vivo muscle engineered tissue’s (X-MET) functional remodelling

P.17  Valeria Runfola (Milan, Italy)
Identification of MATR3 as endogenous inhibitor of DUX4 for the treatment of FSHD muscular dystrophy

P.18  Francesco Palmieri (Florence, Italy)
Role of HIF-1α/MMP-9 axis in promoting skeletal myoblast differentiation under normoxia condition

P.19  Caterina Boccia (Rome, Italy)
Targeting IL-6 signaling to stabilize the dystrophic muscle

P.20  Cristina Purcaro (Chieti, Italy)
Environmental pollutants and muscle stem cells: findings of potential interactions

P.21  Silvia Casati (Milan, Italy)
Targeting mitochondrial dynamics to tackle duchenne muscular dystrophy progression

P.22  Lucia Rossi (Padua, Italy)
Investigating human neuromuscular organoid integration in an engineered skeletal muscle tissue model

P.23  Silvia Codenotti (Brescia, Italy)
The Src/Akt1/caveolin-1/catalase signaling axis is critical for radioresistance of rhabdomyosarcoma cells

P.24  Stefano Testa (Marseille, France)
Development of an innovative extrusion-based 3D bioprinting system and its characterization for Skeletal Muscle Tissue Engineering applications

P.25  Raffaele Epis (Milan, Italy)
Screening of fluorinated compounds to inhibit Nfix in muscular dystrophy

P.26  Alessandro Antonioli (Novara, Italy)
Ad libitum ketogenic diet reverts western diet-pathological effects in liver, but not in skeletal muscle in mice

P.27  Francesca Gasparella (Padua, Italy)
Elucidating the effects of MAO-B inhibitors in DMD: rasagiline treatment in mdx mice dampens the expression of pro-inflammatory genes in multiple cell types

P.28  Mara Barone (Milan, Italy)
Exploring the mechanisms behind gender difference in muscle wasting during cancer cachexia: insights from two animal models

P.29  Gaia Laurenzi (Rome, Italy)
SOD1^G93AKI, a novel ALS mouse model to genetically recapitulate human disease

P.30  Sara Chiappalupi (Perugia, Italy)
Effects of real microgravity on human muscle precursor cells and skeletal muscle tissue. The MyoGravity project

P.31  Paola Mantuano (Bari, Italy)
Antifibrotic potential of growth hormone secretagogues in Duchenne muscular dystrophy: effects of JMV2894 in the D2-mdx mouse model

P.32  Pietro Chiolerio (Padua, Italy)
Human neuromuscular organoids for studying cancer cachexia

P.33  Meriem Matouk (Montigny le Bretonneux, France)
The compartmentalized distribution of proteins of the dystrophin-associated protein complex in the muscle fiber syncytium

P.34  Martina Lunardi (Milan, Italy)
Dissecting gender differences in muscle wasting during cancer cachexia: insights into possible molecular mechanisms

P.35  Maria Pannese (Milan, Italy)
A possible gene therapy approach for FSHD muscular dystrophy

P.36  Martina Paiella (Novara, Italy)
Western diet enriched in advanced glycation end-products (AGEs) induces muscle wasting which could be counteracted by Vaccinium macrocarpon extract

P.37  Cristiana Perrotta (Milan, Italy)
What nutraceuticals can do for Duchenne muscular dystrophy: the plumbagin experience

P.38  Federica Palo (Milan, Italy)
Fatty acid binding protein 3, FABP3, has a role in cachexia induced by pancreatic and colon cancers

P.39  Andrea Pirrottina (Rome, Italy)
Development of efficient gene delivery systems for the treatment of Duchenne muscular dystrophy

P.40  Ilaria Piazza (Padua, Italy)
Altered intracellular Ca²⁺ homeostasis underlies castration-induced skeletal muscle atrophy

P.41  Gabriele Rovetta (Milan, Italy)
Development of a high-throughput screening to identify Nfix-modulating drugs as a novel therapy for muscular dystrophy

P.42  Rosanna Piccirillo (Milan, Italy)
MyoRep: a novel reporter system to detect early muscle atrophy in vivo

P.43  Emilia Skafida (Rome, Italy)
Dynamic changes in transcriptional and epigenetic profile of fibro-adipogenic progenitors in dystrophic skeletal muscles and their modulation by epigenetic drugs (HDACi)

P.44  Giacomo Rubini (Turin, Italy)
Immunomodulation via interleukin-4 improves cachexia in C26 tumor-bearing mice

P.45  Giosuè Annibalini (Urbino, Italy)
N-glycosylation inhibition reduced IGF-1 serum level and muscle IGF-1R signalling pathway activation

P.46  Laura Salvadori (Novara, Italy)
Vaccinium macrocarpon restrains myotube atrophy in experimental models mimicking cancer cachexia

P.47  Elena Conte (Bari, Italy)
Insight into the role of calcium homeostasis and store-operated-calcium-entry dysfunction in sarcopenia: effect of supplementation with BCAA-based formulation in aged mice

P.48  Andrea Scircoli (Novara, Italy)
RNAseq analysis revealed a lower inflammation in tumor-bearing mice lacking VDBP resulting in a milder cachectic phenotype

P.49  Evgeniia Motanova (Padua, Italy)
Morphological alterations of human neuromuscular junction in ageing

P.50  Elisabeth Wyart (Turin, Italy)
Role of glucocorticoids in cancer cachexia : focus on lipocalin 2 and iron homeostasis

P.51  Leonardo Sandrini (Brescia, Italy)
Putative contribution of caveolin-1 in satellite cells senescence

P.52  Rachele Agostini (Urbino, Italy)
Extracellular vesicle release and protein cargo are altered by caveolin-1-overexpression and contribute to cancer dissemination in a model of rhabdomyosarcoma

P.53  Alessandra Guidi (Rome, Italy)
Role of PRDM16 in maintaining nuclear integrity and genomic stability in fibro-adipogenic progenitors

P.54  Suvham Barua (Turin, Italy)
Non-Autonomous Muscle Stem Cells mediated defective muscle regeneration in the accelerated ageing Polg Mutator Mouse Model